Update split_libraries_fastq.py in MacQIIME 1.4.0

March 13th, 2012

There is an update to split_libraries_fastq.py to allow it to handle some of the new FASTQ file formats out there. It's amazing how many different formats there are for a FASTQ file!  If you'd like to update to the newest version of split_libraries_fastq.py in MacQIIME 1.4.0 (or 1.4.0a), you can follow the instructions below. The installation package that you run will replace the following files:





and it will create the following new library:



Instructions: How to update split_libraries_fastq.py

In order to follow these instructions, you need to be logged in as a user with administrative privileges (a sudoer).

1. Download this archive file into your Downloads folder (do not decompress with Archive Utility): fix_split_libraries_fastq.tgz

2. Open the Terminal.app

3. cd into the folder into which you downloaded the file.  E.g., if you put it in the Downloads folder:

cd ~/Downloads

4. Extract the file using the tar utility:

tar -xvf fix_split_libraries_fastq.tgz

5. cd into the new extracted directory:

cd fix_split_libraries_fastq

6. Run the installation script:


You will be asked for a password; this is your administrative (sudo) password for your user account. It is needed to copy files into the /macqiime/ subfolders, which are root-owned.

That should do it!  Please let me know if this causes any problems. Thanks!